Application |
Molecular Biology Reagent | Molecular Biology Reagent | Molecular Biology Reagent | Molecular Biology Reagent | Molecular Biology Reagent |
Features and Benefits |
- A concentrated loading buffer designed for DNA gel electrophoresis applications, containing dual tracking dyes for visual monitoring of DNA migration
- Tracking dyes included: Bromophenol Blue, Xylene Cyanol FF
- Dye concentrations optimized for DNA visualization
- Compatible with standard agarose gels
- Composition: Tris-HCl 60mM, EDTA 6mM, Bromophenol Blue Sodioum Salt .12%, Xylene Cyanol FF .12%, Glycerol 30%
- For DNA markers or ladders: mix one part of the 6x gel loading buffer with five parts of the original DNA ladder, and load into the well of your gel
- For DNA samples: if needed, dilute your DNA samples with TE buffer or water to a desired concentration, then mix one part of the 6x gel loading buffer with five parts of your diluted DNA samples, and load into the well of your gel
- This Product Is For Research Use Only
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- Manufacturer: Invitrogen™ AM9861 - Nuclease-free, ultrapure solution for RNA samples
- TE Buffer consists of 10 mM Tris (adjusted to the specified pH with HCl) and 1 mM EDTA
- These TE Buffers are filtered through a 0.2 μm filter into a sterile autoclavable bottle, are nuclease-free, and ready for use without additional preparation
- Use TE Buffer pH 7.0 and 8.0 in critical molecular biology applications, including resuspension of nucleic acids after precipitation
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- A precision molecular weight marker containing 9 distinct DNA fragments ranging from 100-1500 base pairs (bp)
- Designed for accurate size determination and quantitation of small DNA fragments during gel electrophoresis analysis
- Loading concentration: 540 ng/6 µl
- Quantitative band intensity correlation
- Pre-mixed with loading buffer
- Loading buffer composition: 10mM Tris-HCI, 1 mM EDTA (pH 8.0), 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 5% Glycerol
- Store at -20°C
- For Research Use Only
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- The 1Kb Plus™ DNA Ladder-20 is a comprehensive molecular weight marker containing 14 precisely sized DNA fragments ranging from 100bp to 20kb
- This ready-to-use ladder features enhanced intensity reference bands at 1kb, 3kb, 6kb, and 20kb positions for simplified molecular weight determination
- Ready-to-load format
- Minimum 30ng DNA per band with 5 µL loading
- Ideal size range for most molecular biology applications
- Loading buffer composition: 10mM Tris-HCl, 1mM EDTA (pH 8.0), 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 5% Glycerol
- Store at -20°C
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- 100bp Plus DNA Ladder consists of 13 DNA fragments ranging in size from 100-3000 base pairs (bp)
- 6 µl will yield at least 30ng DNA in any single band
- The intensity of the 500bp and 1500bp bands has been increased to serve as a reference for easy identification
- Volume: 1000 µl
- Loading Buffer Components: 10 mM Tris-HCl, 1 mM EDTA (pH 8.0), 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 5% Glycerol
- Store at -20°C
- For Research Use Only
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